According to the Department of the Treasury:

“The largest denomination of currency ever printed by the Bureau of Engraving and Printing (BEP) was the $100,000 Series 1934 Gold Certificate featuring the portrait of President Wilson. These notes were printed from December 18, 1934 through January 9, 1935 and were issued by the Treasurer of the United States to Federal Reserve Banks only against an equal amount of gold bullion held by the Treasury Department. The notes were used only for official transactions between Federal Reserve Banks and were not circulated among the general public.”

Production of $500, $1,000, $5,000 and $10,000 bills had ceased during World War II, and in 1969 the Department stopped distributing those denominations.

The only U.S. currency denominations in production today are the $1, $2, $5, $10, $20, $50 and $100 bills.